Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: Primers designed for qRT-PCR validation of selected circRNAs. Tm: temperature. bp: base pair

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques: Biomarker Discovery

Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: Top 20 significantly up-regulated circRNAs by microarray analysis.

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques: Microarray

Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: Top 20 significantly down-regulated circRNAs by microarray analysis.

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques: Microarray

Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: Detailed annotation for circRNA-miRNA interaction. A. Top 5 predicted targets of mmu_circRNA_011180, mmu_circRNA_016108, mmu_circRNA_22546 and mmu_circRNA_002573. B. Predicted interaction sites of mmu_circRNA_002573. C. Predicted interaction sites of mmu_circRNA_016108. M: be predicted by miRanda; T: be predicted by TargetScan.

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: The predicted circRNA‐miRNA‐mRNA networks. The red color and light-blue color represent miRNA and mRNA, respectively. Yellow color and green color represent up-regulated and down-regulated circRNAs, respectively. Edges with T-shape arrow represent directed relationships, while edges without arrow represent undirected relationships.

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Identifying circRNA-associated-ceRNA networks in retinal neovascularization in mice

doi: 10.7150/ijms.35149

Figure Lengend Snippet: GO and KEGG pathway analyses of validated circRNAs. For A-C, parental genes according to the values in the enrichment score (left) and gene count (right) under the themes of biological processes, cellular components and molecular functions. The x‐ and y‐axis represent the top 10 significantly enriched terms. A represents biological processes. B represents cellular components. C represents molecular functions. D. The top enriched KEGG pathways of the significantly altered circRNA parental genes.

Article Snippet: Labeled cRNAs were hybridized onto Mouse circRNA Array V2 (8x15K, Arraystar).

Techniques:

Journal: Cell Death & Disease

Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

doi: 10.1038/s41419-019-1590-5

Figure Lengend Snippet: a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h

Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

Techniques: Expressing, Control, Amplification, Marker, Sequencing, Fluorescence, In Situ Hybridization, Staining, Quantitative RT-PCR

Journal: Cell Death & Disease

Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

doi: 10.1038/s41419-019-1590-5

Figure Lengend Snippet: The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions

Article Snippet: Circular RNA expression profiling was performed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, USA).

Techniques: Staining

Journal: Communications Biology

Article Title: Circular RNA cVIM promotes hepatic stellate cell activation in liver fibrosis via miR-122-5p/miR-9-5p-mediated TGF-β signaling cascade

doi: 10.1038/s42003-024-05797-3

Figure Lengend Snippet: Masson staining was performed in CCl 4 mice ( a ) and BDL mice ( b ), n = 6 mice per group. Scale bar, 100 μm. c Heat map for differentially expressed circRNAs analyzed by circRNA arraystar ChIP between the fibrotic tissues and the control tissues ( n = 3 per group). Expression of cVIM in the fibrotic tissues in CCl 4 ( d ) and BDL mice ( e ), n = 6 mice per group. f Expression of cVIM in primary HSCs isolated from healthy mice during culture days ( n = 6 per group). Each value is the mean ± SD of six independent experiments. ** P < 0.01 compared to the control.

Article Snippet: To examine liver circRNAs that were differentially expressed between CCl 4 -treated mice ( n = 3) and healthy control mice ( n = 3), we employed the Arraystar Mouse circRNA Array V2 (manufactured by KangChen Bio-tech in Shanghai, China).

Techniques: Staining, Expressing, Isolation

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop

doi: 10.1002/jcsm.12859

Figure Lengend Snippet: Dynamic expression of circRNAs during pig skeletal muscle development. ( A ) The number of detected circRNAs during each developmental stage. ( B ) PCA plot showing the global view of dynamic circRNA expression during skeletal muscle development, coloured according to developmental stage. The 3382 circRNAs that were expressed in at least 80% of samples were used for PCA analysis. ( C ) Heatmap showing the expression pattern of differentially expressed circRNAs (DECs) during skeletal muscle development. ( D ) Comparison of the transcript length between DECs and non‐DECs. ( E ) Comparison of the exon number between DECs and non‐DECs. ( F ) Dendrogram from WGCNA co‐expression network analysis of skeletal muscle samples. Modules of co‐expressed genes were assigned a colour and number (M1–M12). ( G – I ) GO enrichment analysis of the host genes of DECs in the M2 ( G ), M3 ( H ), and M6 ( I ) modules. The top 10 biological processes reported by DAVID 6.8 are shown.

Article Snippet: The circRNA microarray was analysed using the Arraystar Mouse circRNA Array V2 (Arraystar, Rockville, MD, USA).

Techniques: Expressing, Comparison